Munich experts to discuss novel in vitro cell models

03.2012 – More than 20 scientists met in early March at IZB Martinsried near Munich to discuss ways of further improving in vitro cell models. Modern preclinical research would not anymore exist without them.
Key issues remain with regard to predictivity as there continues to be a lack of advanced cell lines resembling in vivo conditions. Instead, easy to handle cancer cell lines are often being used. Also, there are limitations with traditional 2D cell cultures.
Precise knockdowns approaching 100% are needed. Sophistication in shRNA validation now allows for the identification of highly effective sequences to reach such high levels. Also, clear cut homogeneous overexpressions are now possible with improved and optimized lentiviral vectors. Cell models in 3D are turning into a standard laboratory procedure as they reflect the living organisms significantly better. 3D microtissue generation scaffold free allows for automation with size variation of less than 9% and hence, for high throughput applications in compound screening.
The seminar on 6 March 2012 was hosted by SIRION Biotech GmbH with a speaker from InSphero AG, Zurich.

SIRION BIOTECH GmbH is a biotech company that produces genetically modified cells and is a technology provider in the area of viral vector systems. The company was founded in 2006 and is located in Martinsried near Munich. The expert in RNAi technology offers a wide range of more than 100 products and services. Using state of the art techniques and assay systems the company offers reliable lead-through service projects in target validation, screening and drug discovery.
With its strong expertise in cell line development SIRION BIOTECH serves as a partner for the development and optimization of cell lines for various applications like cellular assay systems and the production of vaccines or antibodies.
SIRION BIOTECH currently operates projects for most of the major pharmaceutical companies in Europe and has ongoing collaborations with leading academic and governmental research institutes.

Related posts:

1. AdenoONE(TM) makes working with adenovirus a standard procedure Adenovirus vector cloning and production still remain tricky with rather low yields and limited success. The brand new AdenoONE(TM) line of support kits turns cloning & production into an easy to schedule project step of less than 4 weeks. AdenoONE(TM) comprises 2 discrete kits: AdenONE(TM) Cloning Kit and AdenoONE(TM) Purification Kit. The latter allows for reliable purification within just 20 minutes. Soon will SIRION BIOTECH, also referred to as Munich-s "Cell Competence Center"...
2. Using Roche-s CASY Cell Counter and Analyzer for proliferation and viability measurement in cancer studies In a recent study (1), Prokop et al. used the CASY Cell Counter and Analyzer from Roche Applied Science (SIX: RO, ROG; OTCQX: RHHBY) to identify the specific anticancer properties of a therapeutic substance in Burkitt-like lymphoma cells (BJAB cells). In addition to using the CASY system for multi-parameter quality control of the cell cultures, the team also brought the system to bear in examining apoptosis induction as early as 24 hours after treatment, i.e., before taking final measurements w...
3. Airvana to Demonstrate LTE Small Cell Technology Airvana to Provide Private Viewing of LTE Small Cell Technology at Mobile World Congress...
4. Roche-s xCELLigence MP Instrument Used in the First Human Kinome Screen for Dynamic RNA Inhibitor Phenotypes during Cell Proliferation 07.2011 - RNA interference studies are a powerful technology for high-throughput screening. However, endpoint analyses only provide a snapshot of the analyzed cellular phenotype, as it is not possible to measure developmental changes over time. To address this, a research team led by Stefan Wiemann and Ulrich Tschulena in the Division Molecular Genome Analysis of the German Cancer Research Center (DKFZ) in Heidelberg, Germany used the xCELLigence MP Instrument, from Roche (SIX: RO, ROG; OTCQX: R...
5. Airvana Launches New Small Cell Analytics Service Airvana Provides Wireless Operators With Customer-Centric View of Femtocell Experience...

Posted by Publisher on 19. March 2012. Filed under Electronics & Semiconductors, Picture Gallery. You can follow any responses to this entry through the RSS 2.0. You can leave a response or trackback to this entry